When it comes to lateral filtration for plasma separation, the key physical characteristics of fiber-based materials that need to be considered to get good and sufficient quantities of plasma are the absorption capacity linked to the thickness of the plasma separation pad, the red blood cell retention capacity and the overall dimension of the pad for the sample volume.
For a fixed volume of blood, as thickness of blood separation pads increases, amount of recovered plasma decreases. However, by using appropriate pads dimensions and a specific chemical treatment, significant improvement in plasma recovery can be achieved.
If the correct fibrous components are not chosen, hemolysis or rupturing of the red blood cells can occur. Hemolysis of any type should be limited within the diagnostic tests, as the rupture of the cells leads to the release of hemoglobin molecules, causing the serum or plasma to have a pink to red color, thus making the readout difficult to judge.
Leakage of the cells content along the membrane can also cause problems such as low sensitivity, selectivity and inaccurate results. One of the main reasons causing hemolysis can be the use of an inappropriate type of glass separator pads, especially the ones with small diameter glass fibers.
Ahlstrom-Munksjö original single layer blood separator, CytoSep®, was developed in early 1990’s to address industry demand for whole blood testing. Working closely with our customer base we continued to develop improved fiber-based materials and methodically upgrade the performance of CytoSep®, meeting the increasing demand for sensitivity, consistency and non-interference. CytoSep® is widely used for rapid separation of plasma from whole blood samples in lateral flow applications, retaining blood cells while allowing serum to flow rapidly.
There are three historical grades which differ in thickness: CytoSep® 1663, CytoSep® 1662, CytoSep® 1660. These untreated media contains no chemical-interfering substances and show no significant binding of plasma components.
Going into our 4th decade of developing improved and innovative components for Lateral Flow Immuno Assays, we continue to lead the industry providing new materials capable of keeping pace with this rapidly evolving industry.
The newest CytoSep® products, HV 1667 and chemically treated HV Plus 1668, are designed to separate increased volumes of whole blood within the same area of media while limiting hemolysis.
CytoSep® HV plus 1668 combines both chemical and mechanical filtration processes for higher performance. The chemicals help to deform shape of red blood cells and so limit their diffusion while the specific pore size blocks the residual mobile RBC. On a defined sample pad area, CytoSep® HV plus 1668 is able to accept 25% more in whole blood volume compare to traditional plasma separation pads.
- Faster blood absorption and plasma separation
- Higher plasma yield for increases in line sensitivity
- Designed with hemolysis prevention in mind
- Ability to separate larger volume blood samples so that less material can be used in new designs
More blood – more plasma – more analytes – higher sensitivity
Discover more about Ahlstrom-Munksjö CytoSep® and other plasma separation pads: https://www.ahlstrom-munksjo.com/products/medical-life-sciences-and-laboratory/lateral-flow-test-pads/plasma-separation-pads/